Multiple assembly states of lumazine synthase: a model relating catalytic function and molecular assembly.

Zhang X, Konarev PV, Petoukhov MV, Svergun DI, Xing L, Cheng RH, Haase I, Fischer M, Bacher A, Ladenstein R, Meining W, J Mol Biol 362(4):753-70 (2006) Europe PMC

SASDAQ5 – Lumazine Synthase

Lumazine Synthase
MWexperimental 960 kDa
MWexpected 960 kDa
VPorod 1450 nm3
log I(s) 5.81×105 5.81×104 5.81×103 5.81×102
Lumazine Synthase small angle scattering data  s, nm-1
ln I(s)
Lumazine Synthase Guinier plot ln 5.82×105 Rg: 6.2 nm 0 (6.2 nm)-2 s2
Lumazine Synthase Kratky plot 1.104 0 3 sRg
Lumazine Synthase pair distance distribution function Rg: 6.2 nm 0 Dmax: 15.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Lumazine Synthase DAMMIN model

Synchrotron SAXS data from solutions of Lumazine Synthase in Borate buffer, pH 7 were collected on the EMBL X33 beam line at the DORIS III storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 15°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Lumazine Synthase (Lumazine Synthase)
Mol. type   Protein
Organism   Bacillus subtilis
Olig. state   Other
Mon. MW   16.3 kDa
Sequence   FASTA