| MWI(0) | 150 | kDa |
| MWexpected | 65 | kDa |
| VPorod | 250 | nm3 |
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log I(s)
1.61×104
1.61×103
1.61×102
1.61×101
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s, nm-1
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KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA.
Ponnusamy R, Petoukhov MV, Correia B, Custodio TF, Juillard F, Tan M, Pires de Miranda M, Carrondo MA, Simas JP, Kaye KM, Svergun DI, McVey CE, Nucleic Acids Res 43(20):10039-54 (2015) Europe PMCSASDAS8 – kLANA 1008-1150 -- kLBS1-2 complex 8:2 (partially dissociated)
kLBS1-2 DNAORF73 tetramer
ORF73 octamer
kLBS1-2 DNA two monomers
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Fits and models
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Synchrotron SAXS
data from solutions of
kLANA 1008-1150 -- kLBS1-2 complex 8:2 (partially dissociated)
in
25 mM Na/K Phosphate, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 0.40 mg/ml was measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Study of oligomeric states of mLANA and kLANA and their complexes with DNA |
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