Dissecting NGF interactions with TrkA and p75 receptors by structural and functional studies of an anti-NGF neutralizing antibody.

Covaceuszach S, Cassetta A, Konarev PV, Gonfloni S, Rudolph R, Svergun DI, Lamba D, Cattaneo A, J Mol Biol 381(4):881-96 (2008) Europe PMC

SASDAT5 – NGF

NGF
MWexperimental 54 kDa
MWexpected 50 kDa
VPorod 102 nm3
log I(s) 1.15×106 1.15×105 1.15×104 1.15×103
NGF small angle scattering data  s, nm-1
ln I(s)
NGF Guinier plot ln 1.16×106 Rg: 3.1 nm 0 (3.1 nm)-2 s2
(sRg)2I(s)/I(0)
NGF Kratky plot 1.104 0 √3 sRg
p(r)
NGF pair distance distribution function Rg: 3.1 nm 0 Dmax: 12 nm

Data validation

Fits and models

log I(s)
 s, nm-1
NGF DAMMIN model
Synchrotron SAXS data from solutions of NGF in 10 mM Na-phosphate 150 mM NaCl, pH 7.8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 15°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN. Concentration = UNKNOWN

Tags: X33
NGF (NGF)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   25 kDa
Sequence   FASTA