The central portion of factor H (modules 10-15) is compact and contains a structurally deviant CCP module.

Schmidt CQ, Herbert AP, Mertens HD, Guariento M, Soares DC, Uhrin D, Rowe AJ, Svergun DI, Barlow PN, J Mol Biol 395(1):105-22 (2010) Europe PMC

SASDAY4 – fH1015

Factor H CCP modules 10 to 15
MWI(0) 46 kDa
MWexpected 41 kDa
VPorod 68 nm3
log I(s) 1.62×100 1.62×10-1 1.62×10-2 1.62×10-3
Factor H CCP modules 10 to 15 small angle scattering data  s, nm-1
ln I(s)
Factor H CCP modules 10 to 15 Guinier plot ln 1.62×100 Rg: 3 nm 0 (3 nm)-2 s2
(sRg)2I(s)/I(0)
Factor H CCP modules 10 to 15 Kratky plot 1.104 0 √3 sRg
p(r)
Factor H CCP modules 10 to 15 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Factor H CCP modules 10 to 15 DAMMIF model
log I(s)
 s, nm-1
Factor H CCP modules 10 to 15 GASBOR model
log I(s)
 s, nm-1
Factor H CCP modules 10 to 15 BUNCH model
log I(s)
 s, nm-1
Factor H CCP modules 10 to 15 BUNCH model
Synchrotron SAXS data from solutions of fH1015 in 50 mM Potassium Phosphate, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.4 and 10.5 mg/ml were measured at 10°C. One 120 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
Factor H CCP modules 10 to 15 (fH1015)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   40.9 kDa
Sequence   FASTA