| MWexperimental | 60 | kDa |
| MWexpected | 60 | kDa |
| VPorod | 90 | nm3 |
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log I(s)
1.14×102
1.14×101
1.14×100
1.14×10-1
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s, nm-1
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The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP, Nucleic Acids Res 45(13):8064-8078 (2017) Europe PMCSASDB48 – Major viral transcription factor ICP4/Immediate Early 3 DNA (ICP4N IE3_19mer) complex
Major viral transcription factor ICP4Immediate Early 3
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Fits and models
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Synchrotron SAXS data from solutions of Major viral transcription factor ICP4/Immediate Early 3 DNA (ICP4N IE3_19mer) complex in 20 mM HEPES, 150 mM NaCl, pH 7.4 were collected using HPLC SEC-SAXS at the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The sample was separated using a Shodex KW-403 SEC column and Agilent HPLC before exposure to X-rays to isolate the ICP4N IE3_19mer complex from any dissociated monomer. 50 µL of ICP4N•IE3_19mer complex was loaded onto the Shodex column and the eluent was flowed through the SAXS beam at 0.15 mL/min; the buffer used as the background was collected after one SEC column volume. Data were collected at 20°C at one second intervals.
Number of frames = UNKNOWN. Concentration min = UNKNOWN. Concentration max = UNKNOWN |
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