The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.

Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP, Nucleic Acids Res 45(13):8064-8078 (2017) PubMed

SASDB58 – Major viral transcription factor ICP4 (ICP4N dimer)

Major viral transcription factor ICP4
MWexperimental 51 kDa
MWexpected 49 kDa
log I(s) 9.33×101 9.33×100 9.33×10-1 9.33×10-2
Major viral transcription factor ICP4 small angle scattering data  s, nm-1
ln I(s)
Major viral transcription factor ICP4 Guinier plot ln 9.33×101 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Major viral transcription factor ICP4 Kratky plot 1.104 0 3 sRg
p(r)
Major viral transcription factor ICP4 pair distance distribution function Rg: 3.1 nm 0 Dmax: 12.7 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Major viral transcription factor ICP4 (ICP4N dimer) in 20 mM HEPES, 150 mM NaCl, pH 7.4, were collected at the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Data were collected at 20°C using 17 successive 10 second frames at an undetermined sample concentration. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Major viral transcription factor ICP4 (ICP4N)
Mol. type   Protein
Organism   Human herpesvirus 1 (strain 17)
Olig. state   Dimer
Mon. MW   24.3 kDa
 
UniProt   P08392 (258-487)
Sequence   FASTA