The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.

Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP, Nucleic Acids Res 45(13):8064-8078 (2017) PubMed

SASDB68 – Immediate Early 3 DNA (ICP4N IE3_19mer)

Immediate Early 3
MWexpected 12 kDa
log I(s) 4.78×101 4.78×100 4.78×10-1 4.78×10-2
Immediate Early 3 small angle scattering data  s, nm-1
ln I(s)
Immediate Early 3 Guinier plot ln 4.79×101 Rg: 1.9 nm 0 (1.9 nm)-2 s2
(sRg)2I(s)/I(0)
Immediate Early 3 Kratky plot 1.104 0 3 sRg
p(r)
Immediate Early 3 pair distance distribution function Rg: 1.9 nm 0 Dmax: 6.9 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Immediate Early 3 DNA (ICP4N IE3_19mer) in 20 mM HEPES, 150 mM NaCl, pH 7.4, were collected using HPLC SEC-SAXS at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). The sample was separated using a Superdex 200 increase 3.2/300 size exclusion chromatography column before exposure to X-rays. 50 µL of IE3_19merwas loaded onto the Superdex column and the eluent was flowed through the SAXS beam at 0.075 mL/min; the buffer used as the background was collected after one SEC column volume. Data were collected at 20°C at one second intervals.

Immediate Early 3 (IE3)
Mol. type   DNA
Olig. state   Dimer
Mon. MW   5.8 kDa
Sequence   FASTA