Visualizing single-stranded nucleic acids in solution.

Plumridge A, Meisburger SP, Pollack L, Nucleic Acids Res 45(9):e66 (2017) PubMed

SASDBD6 – Single stranded poly-deoxythymidine DNA (30mer, dT30)

Poly-deoxythymidine (30mer)
MWexpected 9 kDa
log I(s) 5.02×101 5.02×100 5.02×10-1 5.02×10-2
Poly-deoxythymidine (30mer) small angle scattering data  s, nm-1
ln I(s)
Poly-deoxythymidine (30mer) Guinier plot ln 5.02×101 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Poly-deoxythymidine (30mer) Kratky plot 1.104 0 3 sRg
p(r)
Poly-deoxythymidine (30mer) pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.7 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Single stranded poly-deoxythymidine DNA (30mer, dT30) Rg histogram Rg, nm
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model
Poly-deoxythymidine (30mer) CUSTOM IN-HOUSE model

Synchrotron SAXS data from solutions of single stranded poly-deoxythymidine DNA (30mer, dT30) in 1mM Na MOPS, 20mM NaCl, pH 7 were collected on the G1 Beamline camera on the storage ring CHESS (Ithaca, USA) using a CCD Finger Lakes CCD detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 1.8 mg/ml were measured . 20 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for sample concentration. The low angle data collected at lower concentrations were merged with the higher concentration data to yield the final composite scattering curve.

Poly-deoxythymidine (30mer) (dT30)
Mol. type   DNA
Olig. state   Monomer
Mon. MW   9.1 kDa
Sequence   FASTA