Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis

Beckham K, Ciccarelli L, Bunduc C, Mertens H, Ummels R, Lugmayr W, Mayr J, Rettel M, Savitski M, Svergun D, Bitter W, Wilmanns M, Marlovits T, Parret A, Houben E, Nature Microbiology 2017 Apr 10 2:17047 DOI

SASDBE7 – ESX-5 type VII secretion system protein EccC5

ESX-5 type VII secretion system protein EccC5
MWI(0) 141 kDa
MWexpected 142 kDa
VPorod 255 nm3
log I(s) 1.07×10-1 1.07×10-2 1.07×10-3 1.07×10-4
ESX-5 type VII secretion system protein EccC5 small angle scattering data  s, nm-1
ln I(s)
ESX-5 type VII secretion system protein EccC5 Guinier plot ln 1.07×10-1 Rg: 6.2 nm 0 (6.2 nm)-2 s2
(sRg)2I(s)/I(0)
ESX-5 type VII secretion system protein EccC5 Kratky plot 1.104 0 3 sRg
p(r)
ESX-5 type VII secretion system protein EccC5 pair distance distribution function Rg: 6.5 nm 0 Dmax: 23 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
ESX-5 type VII secretion system protein EccC5 Rg histogram Rg, nm
ESX-5 type VII secretion system protein EccC5 NONE model
ESX-5 type VII secretion system protein EccC5 NONE model
ESX-5 type VII secretion system protein EccC5 NONE model
ESX-5 type VII secretion system protein EccC5 NONE model
ESX-5 type VII secretion system protein EccC5 NONE model
ESX-5 type VII secretion system protein EccC5 NONE model
ESX-5 type VII secretion system protein EccC5 NONE model
log I(s)
 s, nm-1
ESX-5 type VII secretion system protein EccC5 DAMMIN model
Synchrotron SAXS data from solutions of ESX-5 type VII secretion system protein EccC5 in 20 mM Tris 200 mM NaCl 5% (v/v) glycerol, pH 8, were collected at the EMBL-P12 bioSAXS beam line on the PETRA-III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4π sin θ/λ, and 2θ is the scattering angle). A solute concentration of 0.4 mg/ml was measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the resulting subtracted profile normalized to protein concentration.

Note: The data set presented in this entry was measured from is single fraction from an offline size-exclusion chromatography (SEC) purification.

ESX-5 type VII secretion system protein EccC5 (EccC5)
Mol. type   Protein
Organism   Mycobacterium xenopi RIVM700367
Olig. state   Monomer
Mon. MW   142.5 kDa
 
UniProt   I0RZI0
Sequence   FASTA