Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.

Li L, Banerjee A Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV, Mol Microbiol 105(5):777-793 (2017) Europe PMC

SASDBF2 – Solution structure of 40bp long dsDNA-Sa Oligonucleotide

40bp long dsDNA-Sa Oligonucleotide
MWexperimental 82 kDa
MWexpected 25 kDa
log I(s) 8.20×101 8.20×100 8.20×10-1 8.20×10-2
40bp long dsDNA-Sa Oligonucleotide small angle scattering data  s, nm-1
ln I(s)
40bp long dsDNA-Sa Oligonucleotide Guinier plot ln 8.20×101 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
40bp long dsDNA-Sa Oligonucleotide Kratky plot 1.104 0 √3 sRg
p(r)
40bp long dsDNA-Sa Oligonucleotide pair distance distribution function Rg: 3.6 nm 0 Dmax: 12 nm

Data validation

Fits and models

log I(s)
 s, nm-1
40bp long dsDNA-Sa Oligonucleotide DAMMIN model
X-ray synchrotron radiation scattering data from solutions of SaOligo (40bp long dsDNA) in 0.5 x TBE was collected on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). A single 1 mg/mL concentration was measured. 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT).

40bp long dsDNA-Sa Oligonucleotide (dsDNA-Sa Oligo)
Mol. type   DNA
Olig. state   Monomer
Mon. MW   24.7 kDa
Sequence   FASTA