Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius

Li L, Banerjee A, Franziska Bischof L, Ramadan Maklad H, Hoffmann L, Henche A, Veliz F, Bildl W, Schulte U, Orell A, Essen L, Peeters E, Albers S, Molecular Microbiology 2017 Jun 19 DOI

SASDBF2 – Solution structure of 40bp long dsDNA-Sa Oligonucleotide

40bp long dsDNA-Sa Oligonucleotide
MWexperimental 82 kDa
MWexpected 25 kDa
log I(s) 8.20×101 8.20×100 8.20×10-1 8.20×10-2
40bp long dsDNA-Sa Oligonucleotide small angle scattering data  s, nm-1
ln I(s)
40bp long dsDNA-Sa Oligonucleotide Guinier plot ln 8.20×101 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
40bp long dsDNA-Sa Oligonucleotide Kratky plot 1.104 0 3 sRg
p(r)
40bp long dsDNA-Sa Oligonucleotide pair distance distribution function Rg: 3.6 nm 0 Dmax: 12 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
40bp long dsDNA-Sa Oligonucleotide DAMMIN model
X-ray synchrotron radiation scattering data from solutions of SaOligo (40bp long dsDNA) in 0.5 x TBE was collected on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). A single 1 mg/mL concentration was measured. 10 successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT).

40bp long dsDNA-Sa Oligonucleotide (dsDNA-Sa Oligo)
Mol. type   DNA
Olig. state   Monomer
Mon. MW   24.7 kDa
Sequence   FASTA