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13 hits found for Banerjee

SASDBZ3 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage)/DNA complex (light state)

Aureobox dsDNAAureochrome 1a bZIP-LOV module experimental SAS data
DAMFILT model
Sample: Aureobox dsDNA monomer, 13 kDa synthetic construct DNA
Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer: 50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
RgGuinier 4.5 nm
Dmax 16.7 nm
VolumePorod 97 nm3

SASDB24 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state, Tris)

Aureochrome 1a bZIP-LOV module experimental SAS data
Aureochrome 1a bZIP-LOV module Kratky plot
Sample: Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer: 10 mM Tris 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 121 nm3

SASDB34 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (dark state, Tris)

Aureochrome 1a bZIP-LOV module experimental SAS data
Aureochrome 1a bZIP-LOV module Kratky plot
Sample: Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer: 10 mM Tris 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
RgGuinier 3.8 nm
Dmax 12.5 nm
VolumePorod 117 nm3

SASDBD2 – Dark state solution structure of untagged Aureochrome1a- A´α-LOV-Jα

Aureochrome1a- A´α-LOV-Jα (Dark State) experimental SAS data
DAMMIN model
Sample: Aureochrome1a- A´α-LOV-Jα (Dark State) dimer, 32 kDa Phaeodactylum tricornutum protein
Buffer: 10 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2015 Sep 9
Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Structure 24(1):171-178 (2016)
Banerjee A, Herman E, Kottke T, Essen LO
RgGuinier 2.7 nm
Dmax 9.2 nm
VolumePorod 58 nm3

SASDBE2 – Light state solution structure of untagged Aureochrome1a- A´α-LOV-Jα

Aureochrome1a- A´α-LOV-Jα (Light State) experimental SAS data
DAMMIN model
Sample: Aureochrome1a- A´α-LOV-Jα (Light State) dimer, 32 kDa Phaeodactylum tricornutum protein
Buffer: 10 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2015 Sep 9
Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Structure 24(1):171-178 (2016)
Banerjee A, Herman E, Kottke T, Essen LO
RgGuinier 2.6 nm
Dmax 9.8 nm
VolumePorod 66 nm3

SASDBF2 – Solution structure of 40bp long dsDNA-Sa Oligonucleotide

40bp long dsDNA-Sa Oligonucleotide experimental SAS data
DAMMIN model
Sample: 40bp long dsDNA-Sa Oligonucleotide monomer, 25 kDa DNA
Buffer: 0.5 x Tris/Borate/EDTA (TBE), pH:
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 28
Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol 105(5):777-793 (2017)
...Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
RgGuinier 3.6 nm
Dmax 12.0 nm

SASDBG2 – Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).

Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). experimental SAS data
DAMMIN model
Sample: Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer: 20 mM HEPES 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol 105(5):777-793 (2017)
...Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
RgGuinier 2.6 nm
Dmax 9.8 nm
VolumePorod 47 nm3

SASDBH2 – Mutant archaeal biofilm regulator 1 (ABfR1) phosphomimic Y84E S87D

Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant  experimental SAS data
DAMMIN model
Sample: Archaeal biofilm regulator 1 (AbfR1) mutant Y84E S87D phosphomimic mutant dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer: 20 mM HEPES 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol 105(5):777-793 (2017)
...Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
RgGuinier 2.6 nm
Dmax 9.5 nm
VolumePorod 51 nm3

SASDBJ2 – Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide.

40bp long dsDNA-Sa OligonucleotideWild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). experimental SAS data
DAMMIN model
Sample: 40bp long dsDNA-Sa Oligonucleotide monomer, 25 kDa DNA
Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). dimer, 26 kDa Sulfolobus acidocaldarius protein
Buffer: 0.5 x Tris/Borate/EDTA (TBE), pH:
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius. Mol Microbiol 105(5):777-793 (2017)
...Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV
RgGuinier 3.0 nm
Dmax 11.8 nm

SASDAK8 – Dark state solution structure of Aureochrome1a- A´α-LOV-Jα

Aureochrome1a-A´α-LOV-Jα experimental SAS data
DAMMIF model
Sample: Aureochrome1a-A´α-LOV-Jα dimer, 36 kDa Phaeodactylum tricornutum protein
Buffer: 10 mM Tris 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 16
Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Structure 24(1):171-178 (2016)
Banerjee A, Herman E, Kottke T, Essen LO
RgGuinier 3.2 nm
Dmax 13.0 nm
VolumePorod 67 nm3

SASDAJ8 – Light state solution structure of Aureochrome1a- A´α-LOV-Jα

Aureochrome1a-A´α-LOV-Jα experimental SAS data
DAMMIF model
Sample: Aureochrome1a-A´α-LOV-Jα dimer, 36 kDa Phaeodactylum tricornutum protein
Buffer: 10 mM Tris 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 16
Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. Structure 24(1):171-178 (2016)
Banerjee A, Herman E, Kottke T, Essen LO
RgGuinier 3.3 nm
Dmax 13.0 nm
VolumePorod 88 nm3

SASDBX3 – Aureobox dsDNA

Aureobox dsDNA experimental SAS data
DAMFILT model
Sample: Aureobox dsDNA monomer, 13 kDa synthetic construct DNA
Buffer: 50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
RgGuinier 2.0 nm
Dmax 8.6 nm
VolumePorod 18 nm3

SASDBY3 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage) module (light state-TBE)

Aureochrome 1a bZIP-LOV module experimental SAS data
DAMFILT model
Sample: Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
Buffer: 50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2014 Nov 6
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
RgGuinier 3.4 nm
Dmax 12.6 nm
VolumePorod 115 nm3