| MWI(0) | 48 | kDa |
| MWexpected | 51 | kDa |
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log I(s)
4.79×101
4.79×100
4.79×10-1
4.79×10-2
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s, nm-1
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Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.
Li L, Banerjee A Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV, Mol Microbiol 105(5):777-793 (2017) Europe PMCSASDBJ2 – Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide.
40bp long dsDNA-Sa OligonucleotideWild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).
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Fits and models
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X-ray synchrotron radiation scattering data from solutions of N terminal affinity tag cleaved (by thrombin) wild type AbfR1 in complex with SaOligo in 0.5 x TBE buffer was collected on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). A single concentration of 3.5mg/ mL was measured in 10 successive 20 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted .The analysis was performed using the ATSAS package. The displayed model is the best fit model with the lowest reduced Chi-squared value (1.55) after imposing P2 symmetry. The DNA is considerably bent in complex with ABfR1.
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