Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.

Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T, Nucleic Acids Res 44(12):5957-70 (2016) PubMed

SASDBZ3 – Aureochrome 1a bZIP-LOV module: PtAUREO1a bZIP-LOV (Light oxygen voltage)/DNA complex (light state)

Aureobox dsDNA
Aureochrome 1a bZIP-LOV module
MWexperimental 62 kDa
MWexpected 70 kDa
VPorod 97 nm3
log I(s) 6.17×101 6.17×100 6.17×10-1 6.17×10-2
Aureobox dsDNA Aureochrome 1a bZIP-LOV module small angle scattering data  s, nm-1
ln I(s)
Aureobox dsDNA Aureochrome 1a bZIP-LOV module Guinier plot ln 6.18×101 Rg: 4.5 nm 0 (4.5 nm)-2 s2
(sRg)2I(s)/I(0)
Aureobox dsDNA Aureochrome 1a bZIP-LOV module Kratky plot 1.104 0 3 sRg
p(r)
Aureobox dsDNA Aureochrome 1a bZIP-LOV module pair distance distribution function Rg: 4.9 nm 0 Dmax: 16.7 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Aureobox dsDNA Aureochrome 1a bZIP-LOV module DAMFILT model

X-ray synchrotron radiation scattering data from solution of PtAUREO1a bZIP-LOV module in complex with aureo box dsDNA in 0.5X TBE (50 mM Tris pH 8, 50 mM boric acid, 1 mM EDTA) buffer was collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Ten successive 20 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Solution scattering data of complex in the light-state was performed at 1.25mg/mL concentration at 4 degrees centigrade. The modelling analysis was performed using the ATSAS package. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.

Aureobox dsDNA
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   13.0 kDa
Sequence   FASTA
 
Aureochrome 1a bZIP-LOV module (PtAUREO1a bZIP-LOV)
Mol. type   Protein
Organism   Phaeodactylum tricornutum
Olig. state   Dimer
Mon. MW   28.7 kDa
Sequence   FASTA