Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans.

Bhati M, Llamosas E, Jacques DA, Jeffries CM, Dastmalchi S, Ripin N, Nicholas HR, Matthews JM, Sci Rep 2017 Jul 4;7(1):4579 PubMed

SASDC22 – Tandem LIM domains of the neuronal transcription factor homeobox protein CEH-14 fused to the LIM interaction domain of ceLIM-7

Homeobox protein CEH-14
CeLIM-7
MWI(0) 16.7 kDa
MWexpected 19 kDa
VPorod 25.6 nm3
log I(s) 8.30×10-2 8.30×10-3 8.30×10-4 8.30×10-5
Homeobox protein CEH-14 CeLIM-7 small angle scattering data  s, nm-1
ln I(s)
Homeobox protein CEH-14 CeLIM-7 Guinier plot ln 8.30×10-2 Rg: 2.4 nm 0 (2.4 nm)-2 s2
(sRg)2I(s)/I(0)
Homeobox protein CEH-14 CeLIM-7 Kratky plot 1.104 0 3 sRg
p(r)
Homeobox protein CEH-14 CeLIM-7 pair distance distribution function Rg: 2.5 nm 0 Dmax: 8.9 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Homeobox protein CEH-14 CeLIM-7 DAMMIN model
Homeobox protein CEH-14 CeLIM-7 DAMMIF model
log I(s)
 s, nm-1
Homeobox protein CEH-14 CeLIM-7 GASBOR model
log I(s)
 s, nm-1
Homeobox protein CEH-14 CeLIM-7 SWISSMODEL model
SAXS data from solutions of the tandem LIM domains of the neuronal transcription factor homeobox protein CEH-14 fused to the LIM interaction domain of ceLIM-7 in 50 mM Tris, 100 mM NaCl, 5 mM TCEP, pH 8 were collected on an Anton Paar SAXSess 10 mm line collimated instrument (The University of Sydney, Australia) using a CCD Roper Scientific KAF 2084 x 2084 SCX CCD camera at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.1542 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 5.50 mg/ml was measured at 10°C. Four successive 900 second frames were collected. The data were normalized to the intensity of the transmitted beam and integrated; the scattering of the solvent-blank was subtracted to produce the smeared scattering profile of the protein in solution.

The SAXS data displayed for this entry includes the beam-geometry corrected scattering profile (desmeared data; top and homology model fit) as well as the fit of the ab initio models to the smeared SAXS data (i.e., smeared model-data fits). The original smeared data, prepared experimental beam profile and all individual DAMMIF and Gasbor models are included in the full entry zip archive.

Homeobox protein CEH-14 (CEH-14)
Mol. type   Protein
Organism   Caenorhabditis elegans
Olig. state   Monomer
Mon. MW   15.7 kDa
 
UniProt   P20271 (42-168)
Sequence   FASTA
 
CeLIM-7 (CeLIM-7)
Mol. type   Protein
Organism   Caenorhabditis elegans
Olig. state   Monomer
Mon. MW   3.5 kDa
 
UniProt   G5EC36 (347-376)
Sequence   FASTA