| MWexperimental | 42 | kDa |
| MWexpected | 28 | kDa |
| VPorod | 69 | nm3 |
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log I(s)
8.18×102
8.18×101
8.18×100
8.18×10-1
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s, nm-1
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Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C, Structure 26(2):345-355.e5 (2018) Europe PMCSASDC47 – Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)
saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol)
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Fits and models
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Synchrotron SAXS
data from solutions of
Saposin nanoparticle (SapNP) SapA:POPG (SEC-SAXS)
in
PBS, pH 7.4
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 7.10 mg/ml was measured
at 10°C.
3600 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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