Optical and Structural Characterization of a Chronic Myeloid Leukemia DNA Biosensor.

Cordeiro M, Otrelo-Cardoso AR, Svergun DI, Konarev PV, Lima JC, Santos-Silva T, Baptista PV, ACS Chem Biol 13(5):1235-1242 (2018) PubMed

SASDC95 – Light encoded DNA biosensor: e14A DNA

e14A
MWexperimental 22 kDa
MWexpected 21 kDa
log I(s) 6.17×101 6.17×100 6.17×10-1 6.17×10-2
e14A small angle scattering data  s, nm-1
ln I(s)
e14A Guinier plot ln 6.17×101 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
e14A Kratky plot 1.104 0 3 sRg
p(r)
e14A pair distance distribution function Rg: 2.9 nm 0 Dmax: 12.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
e14A DAMMIF model

Synchrotron SAXS data from solutions of Light encoded DNA biosensor: e14A DNA in 154 mM NaCl, pH 8.3 were collected at the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.50 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve. The model depicts the averaged spatial representation of the DNA (DAMFILT occupancy and volume-corrected bead model).

e14A
Mol. type   DNA
Olig. state   Monomer
Mon. MW   21.2 kDa
Sequence   FASTA