SASDCE8 – Beta amylase from sweet potato (WAXS)

Beta-amylase

MW^experimental	157	kDa
MW^expected	224	kDa

log I(s) 8.10×10³ 8.10×10² 8.10×10¹ 8.10×10⁰

Beta-amylase small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 8.10×10³ R_g: 4.0 nm 0 (4.0 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

Data validation

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Beta-amylase PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of beta amylase from sweet potato in tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany), using a Pilatus 2M detector at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 40.10 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled for protein concentration.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Tags: benchmark

Beta-amylase
Mol. type		Protein
Organism		Ipomoea batatas
Olig. state		Tetramer
Mon. MW		56.0 kDa

UniProt		P10537
Sequence		FASTA

PDB ID		1FA2