WAXS benchmark on standard proteins

Maxim Petoukhov.

SASDCE8 – Beta amylase from sweet potato (WAXS)

Beta-amylase
MWexperimental 157 kDa
MWexpected 224 kDa
log I(s) 8.10×103 8.10×102 8.10×101 8.10×100
Beta-amylase small angle scattering data  s, nm-1
ln I(s)
Beta-amylase Guinier plot ln 8.10×103 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Beta-amylase Kratky plot 1.104 0 3 sRg

Data validation


Fits and models


log I(s)
 s, nm-1
Beta-amylase PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of beta amylase from sweet potato in tbs, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany), using a Pilatus 2M detector at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 40.10 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the data were scaled for protein concentration.

Tags: benchmark
Beta-amylase
Mol. type   Protein
Organism   Ipomoea batatas
Olig. state   Tetramer
Mon. MW   56.0 kDa
 
UniProt   P10537
Sequence   FASTA
 
PDB code   1FA2