Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release.

Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC, Nat Commun 8(1):1708 (2017) Europe PMC

SASDCF7 – Dimeric Sortilin at pH 7.4 in the presence of neurotensin

Sortilin, also: Neurotensin-receptor 3
MWexperimental 149 kDa
MWexpected 153 kDa
VPorod 253 nm3
log I(s) 1.24×101 1.24×100 1.24×10-1 1.24×10-2
Sortilin, also: Neurotensin-receptor 3 small angle scattering data  s, nm-1
ln I(s)
Sortilin, also: Neurotensin-receptor 3 Guinier plot ln 1.24×101 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Sortilin, also: Neurotensin-receptor 3 Kratky plot 1.104 0 √3 sRg
p(r)
Sortilin, also: Neurotensin-receptor 3 pair distance distribution function Rg: 3.8 nm 0 Dmax: 13.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Dimeric Sortilin in the presence of neurotensin in 25 mM HEPES, 150 mM NaCl, pH 7.4, were collected at the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). The data were collected at at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames used for averaging: unknown. Exposure time: unknown. Sample concentration: unknown.

Sortilin, also: Neurotensin-receptor 3 (Sort1, also: NTR3)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   76.6 kDa
 
UniProt   Q6PHU5
Sequence   FASTA