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11 hits found for Brennich

SASDCZ5 – Monomeric Sortilin at pH 7.4

Sortilin 1 A464E alias Neurotensin-receptor 3 A464E experimental SAS data
CORAL model
Sample: Sortilin 1 A464E alias Neurotensin-receptor 3 A464E monomer, Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 217 nm3

SASDD93 – ATP-dependent Clp protease ATP-binding subunit ClpC1

ATP-dependent Clp protease ATP-binding subunit ClpC1 experimental SAS data
OTHER model
Sample: ATP-dependent Clp protease ATP-binding subunit ClpC1 unknown, Mycobacterium tuberculosis (strain ... protein
Buffer: Hepes 50 mM pH 7.5, KCl 100 mM, glycerol 10%, MgCl2 4 mM and ATP 1 mM, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 18
The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis. J Biol Chem 293(22):8379-8393 (2018)
...Brennich M, Kazmaier U, Lelievre J, Ballell L, Goldberg A, Schanda P, Fraga H
RgGuinier 7.6 nm
Dmax 25.0 nm
VolumePorod 2156 nm3

SASDDA3 – ATP-dependent Clp protease ATP-binding subunit ClpC1, second state

ATP-dependent Clp protease ATP-binding subunit ClpC1 experimental SAS data
ATP-dependent Clp protease ATP-binding subunit ClpC1 Kratky plot
Sample: ATP-dependent Clp protease ATP-binding subunit ClpC1 unknown, Mycobacterium tuberculosis (strain ... protein
Buffer: Hepes 50 mM pH 7.5, KCl 100 mM, glycerol 10%, MgCl2 4 mM and ATP 1 mM, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 18
The antibiotic cyclomarin blocks arginine-phosphate-induced millisecond dynamics in the N-terminal domain of ClpC1 from Mycobacterium tuberculosis. J Biol Chem 293(22):8379-8393 (2018)
...Brennich M, Kazmaier U, Lelievre J, Ballell L, Goldberg A, Schanda P, Fraga H
RgGuinier 7.9 nm
Dmax 25.1 nm
VolumePorod 2416 nm3

SASDCE7 – Monomeric Sortilin at pH 7.4 in the presence of neurotensin

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
Sortilin, also: Neurotensin-receptor 3 Kratky plot
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.3 nm
Dmax 11.7 nm
VolumePorod 192 nm3

SASDCF7 – Dimeric Sortilin at pH 7.4 in the presence of neurotensin

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
Sortilin, also: Neurotensin-receptor 3 Kratky plot
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.7 nm
Dmax 13.5 nm
VolumePorod 253 nm3

SASDEG2 – Mitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial GTP/GDP carrier protein 1 experimental SAS data
DAMMIF model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 hexamer, Saccharomyces cerevisiae (strain ... protein
Mitochondrial import inner membrane translocase subunit TIM10 hexamer, Saccharomyces cerevisiae (strain ... protein
Mitochondrial GTP/GDP carrier protein 1 monomer, Saccharomyces cerevisiae (strain ... protein
Buffer: 50mM Tris, 150mM NaCl, imidiazole, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P
RgGuinier 4.5 nm
Dmax 16.0 nm
VolumePorod 272 nm3

SASDEF2 – Mitochondrial import inner membrane translocase complex TIM9·10

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CORAL model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, Saccharomyces cerevisiae (strain ... protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, Saccharomyces cerevisiae (strain ... protein
Buffer: 50mM Tris, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDBT4 – Bovine serum albumin monomer (measured by SEC-SAXS)

Bovine serum albumin, monomer experimental SAS data
PDB model
Sample: Bovine serum albumin, monomer monomer, Bos taurus protein
Buffer: 20 mM Tris 142 mM NaCl 5 % Glycerol 1mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Bovine Serum Albumin measured by SEC-SAXS
Martha Brennich
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 118 nm3

SASDCW5 – Dimeric Sortilin at pH 7.4

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
DAMMIF model
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 275 nm3

SASDCX5 – Monomeric Sortilin at pH 5.5

Sortilin 1 A464E alias Neurotensin-receptor 3 A464E experimental SAS data
DAMMIF model
Sample: Sortilin 1 A464E alias Neurotensin-receptor 3 A464E monomer, Mus musculus protein
Buffer: 25 mM MES pH 5.5, 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.4 nm
Dmax 10.0 nm
VolumePorod 217 nm3

SASDCY5 – Dimeric Sortilin at pH 5.5

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
CORAL model
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, Mus musculus protein
Buffer: 25 mM MES pH 5.5, 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
...Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 336 nm3