WAXS benchmark on standard proteins

Maxim Petoukhov.

SASDCF8 – Bovine serum albumin (WAXS)

Serum albumin
MWexperimental 66 kDa
MWexpected 66 kDa
log I(s) 4.46×101 4.46×100 4.46×10-1 4.46×10-2
Serum albumin small angle scattering data  s, nm-1
ln I(s)
Serum albumin Guinier plot ln 4.47×101 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Serum albumin Kratky plot 1.104 0 3 sRg

Data validation


Fits and models


log I(s)
 s, nm-1
Serum albumin PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of bovine serum albumin in HEPES, pH 7.5 were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany), using a Pilatus 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.062 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 17.10 mg/ml was measured at 20°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: benchmark
Serum albumin (BSA)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   66.2 kDa
 
UniProt   P02769 (25-607)
Sequence   FASTA
 
PDB code   3V03