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16 hits found for Serum albumin

SASDA32 – BSA in HEPES

Serum albumin experimental SAS data
DAMMIF model
Sample: Serum albumin monomer, Bos taurus protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III on 2012 Sep 12
Standard proteins
Darja Ruskule
RgGuinier 2.9 nm
Dmax 9.3 nm
VolumePorod 97 nm3

SASDE65 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin monomer, Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 92 nm3

SASDE75 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin monomer, Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 104 nm3

SASDE85 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin monomer, Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 85 nm3

SASDE95 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin monomer, Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.9 nm
Dmax 8.6 nm
VolumePorod 74 nm3

SASDAA6 – ...serum albumin monomer and mixtures

Human serum albumin monomer experimental SAS data
DAMMIF model
Sample: ...serum albumin monomer monomer, Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 22
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 103 nm3

SASDEB5 – ...serum albumin from in-house SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin monomer, Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 May 4
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 99 nm3

SASDEC5 – ...serum albumin dimer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin dimer, Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 4.1 nm
Dmax 13.8 nm
VolumePorod 200 nm3

SASDED5 – ...serum albumin monomer from synchrotron SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin monomer, Bos taurus protein
Buffer: PBS + 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 14
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.7 nm
Dmax 8.7 nm
VolumePorod 84 nm3

SASDEE5 – ...serum albumin from synchrotron SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin monomer, Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 85 nm3

SASDCJ3 – ...Serum Albumin

Serum albumin experimental SAS data
DAMMIN model
Sample: Serum albumin monomer, Bos taurus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, PĂ©rez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...
RgGuinier 2.8 nm
Dmax 8.7 nm
VolumePorod 101 nm3

SASDBK3 – ...serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data
PDB model
Sample: ...serum albumin, dimer dimer, Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 3.9 nm
Dmax 12.7 nm
VolumePorod 202 nm3

SASDDN3 – ...serum albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets)

Bovine serum albumin experimental SAS data
Bovine serum albumin Kratky plot
Sample: ...serum albumin unknown, Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 25
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 117 nm3

SASDEA5 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: ...serum albumin monomer, Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 25
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument Journal of Applied Crystallography 51(6) (2018)
Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.6 nm
VolumePorod 78 nm3

SASDBJ3 – ...serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data
PDB model
Sample: ...serum albumin, monomer monomer, Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 100 nm3

SASDBT4 – ...serum albumin monomer (measured by SEC-SAXS)

Bovine serum albumin, monomer experimental SAS data
PDB model
Sample: ...serum albumin, monomer monomer, Bos taurus protein
Buffer: 20 mM Tris 142 mM NaCl 5 % Glycerol 1mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
...Serum Albumin measured by SEC-SAXS
Martha Brennich
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 118 nm3