Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.

Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH, Structure 23(5):809-818 (2015) Europe PMC

SASDCM3 – Atg17-Atg31-Atg29 Subcomplex

Autophagy-related protein 17
Autophagy-related protein 29
KLLA0A10637p
MWexperimental 152 kDa
MWexpected 152 kDa
VPorod 848 nm3
log I(s) 2.32×103 2.32×102 2.32×101 2.32×100
Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p small angle scattering data  s, nm-1
ln I(s)
Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p Guinier plot ln 2.33×103 Rg: 10.1 nm 0 (10.1 nm)-2 s2
(sRg)2I(s)/I(0)
Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p Kratky plot 1.104 0 3 sRg
p(r)
Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p pair distance distribution function Rg: 10.9 nm 0 Dmax: 34 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Atg17-Atg31-Atg29 Subcomplex in 20 mM Tris, 200 mM NaCl, 2% glycerol, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5.3 mg/ml were measured at 22°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.

Wavelength = UNKNOWN. Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Autophagy-related protein 17 (Atg17)
Mol. type   Protein
Organism   Kluyveromyces lactis
Olig. state   Dimer
Mon. MW   49.9 kDa
 
UniProt   Q6CS99 (1-423)
Sequence   FASTA
 
Autophagy-related protein 29
Mol. type   Protein
Organism   Kluyveromyces lactis
Olig. state   Dimer
Mon. MW   10.0 kDa
 
UniProt   Q6CTU8 (1-85)
Sequence   FASTA
 
KLLA0A10637p (Atg31)
Mol. type   Protein
Organism   Kluyveromyces lactis
Olig. state   Dimer
Mon. MW   16.1 kDa
 
UniProt   Q6CX74 (1-143)
Sequence   FASTA