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6 hits found for Ragusa

SASDC73 – Atg1-Atg13-Atg17-Atg31-Atg29 Minipentamer Complex

Serine/threonine-protein kinase ATG1Autophagy-related protein 13Autophagy-related protein 17Autophagy-related protein 29KLLA0A10637p experimental SAS data
Serine/threonine-protein kinase ATG1 Autophagy-related protein 13 Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p Kratky plot
Sample: Serine/threonine-protein kinase ATG1 dimer, 61 kDa Kluyveromyces lactis protein
Autophagy-related protein 13 dimer, 17 kDa Kluyveromyces lactis protein
Autophagy-related protein 17 dimer, 100 kDa Kluyveromyces lactis protein
Autophagy-related protein 29 dimer, 20 kDa Kluyveromyces lactis protein
KLLA0A10637p dimer, 32 kDa Kluyveromyces lactis protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 20
Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site. Structure 23(5):809-818 (2015)
...Ragusa MJ, Lee IH, Hummer G, Hurley JH
RgGuinier 10.3 nm
Dmax 34.0 nm
VolumePorod 1000 nm3

SASDCL3 – Atg1-Atg13 Subcomplex

Serine/threonine-protein kinase ATG1Autophagy-related protein 13 experimental SAS data
Serine/threonine-protein kinase ATG1 Autophagy-related protein 13 Kratky plot
Sample: Serine/threonine-protein kinase ATG1 dimer, 61 kDa Kluyveromyces lactis protein
Autophagy-related protein 13 dimer, 17 kDa Kluyveromyces lactis protein
Buffer: 20 mM Tris, 200 mM NaCl, 2% glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 10
Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site. Structure 23(5):809-818 (2015)
...Ragusa MJ, Lee IH, Hummer G, Hurley JH
RgGuinier 3.3 nm
Dmax 10.9 nm
VolumePorod 160 nm3

SASDCM3 – Atg17-Atg31-Atg29 Subcomplex

Autophagy-related protein 17Autophagy-related protein 29KLLA0A10637p experimental SAS data
Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p Kratky plot
Sample: Autophagy-related protein 17 dimer, 100 kDa Kluyveromyces lactis protein
Autophagy-related protein 29 dimer, 20 kDa Kluyveromyces lactis protein
KLLA0A10637p dimer, 32 kDa Kluyveromyces lactis protein
Buffer: 20 mM Tris, 200 mM NaCl, 2% glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Dec 10
Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site. Structure 23(5):809-818 (2015)
...Ragusa MJ, Lee IH, Hummer G, Hurley JH
RgGuinier 10.1 nm
Dmax 34.0 nm
VolumePorod 848 nm3

SASDL39 – Autophagy-related protein 23

Autophagy-related protein 23 experimental SAS data
Autophagy-related protein 23 Kratky plot
Sample: Autophagy-related protein 23 dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 3
Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
...Ragusa MJ
RgGuinier 7.4 nm
Dmax 29.3 nm
VolumePorod 378 nm3

SASDL49 – Autophagy-related protein 23 L171A,I182A,L189A mutant

Autophagy-related protein 23 LIL Mutant experimental SAS data
Autophagy-related protein 23 LIL Mutant Kratky plot
Sample: Autophagy-related protein 23 LIL Mutant monomer, 52 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8
Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
...Ragusa MJ
RgGuinier 4.4 nm
Dmax 17.1 nm
VolumePorod 98 nm3

SASDL59 – Autophagy-related protein 23 R190A,K193A,K194A mutant

Autophagy-related protein 23 RKK Mutant experimental SAS data
Autophagy-related protein 23 RKK Mutant Kratky plot
Sample: Autophagy-related protein 23 RKK Mutant dimer, 104 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, 0.2 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 8
Dimerization-dependent membrane tethering by Atg23 is essential for yeast autophagy. Cell Rep 39(3):110702 (2022)
...Ragusa MJ
RgGuinier 7.6 nm
Dmax 34.5 nm
VolumePorod 365 nm3