| MWexperimental | 279 | kDa |
| MWexpected | 227 | kDa |
| VPorod | 511 | nm3 |
|
log I(s)
4.70×102
4.70×101
4.70×100
4.70×10-1
|
s, nm-1
|
Signaling ammonium across membranes through an ammonium sensor histidine kinase.
Pflüger T, Hernández CF, Lewe P, Frank F, Mertens H Svergun D, Baumstark MW, Lunin VY, Jetten MSM, Andrade SLA, Nat Commun 9(1):164 (2018) Europe PMCSASDCM9 – Ammonium transporter Ks-Amt5
ammonium sensor/transducer|
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Fits and models
|
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Synchrotron SAXS
data from solutions of
Ammonium transporter Ks-Amt5
in
20 mM Tris/HCl,100 mM NaCl, 5%(w/v) glycerol, 0.09%(w/v) CYMAL-5, pH 8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 2.7 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
at 10 mg/ml was injected
onto a column
at 10°C.
3600 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
MW estimate from excluded volume (DAMMIN) |
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