Solution Structure Analysis Of An Exchange Protein Directly Activated By cAMP

Mark White.

SASDCQ6 – Rap guanine nucleotide exchange factor 3 (isoform3) - apo form

Rap guanine nucleotide exchange factor 3
MWI(0) 73 kDa
MWexpected 100 kDa
VPorod 180 nm3
log I(s) 2.16×10-1 2.16×10-2 2.16×10-3 2.16×10-4
Rap guanine nucleotide exchange factor 3 small angle scattering data  s, nm-1
ln I(s)
Rap guanine nucleotide exchange factor 3 Guinier plot ln 2.17×10-1 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Rap guanine nucleotide exchange factor 3 Kratky plot 1.104 0 3 sRg
p(r)
Rap guanine nucleotide exchange factor 3 pair distance distribution function Rg: 3.4 nm 0 Dmax: 11 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 SWISSMODEL model

log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 DAMMIF model

SAXS data from solutions of Rap guanine nucleotide exchange factor 3 (isoform3) in 1mM EDTA, 10mM DTT, 500mM NaCl, 10mM Tris, pH 9 were collected using a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) equipped with a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). A sample concentration of 0.5 mg/ml was measured at 10°C. SAXS data were acquired from 12 successive 21600 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to generate the data displayed for this entry.

Rap guanine nucleotide exchange factor 3 (EPAC1B)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   100.0 kDa
 
UniProt   O95398-3 (2-881)
Sequence   FASTA
 
PDB code   4F7Z