Conformational States of Exchange Protein Directly Activated by cAMP (EPAC1) Revealed by Ensemble Modeling and Integrative Structural Biology.

White MA, Tsalkova T, Mei FC, Cheng X, Cells 9(1) (2019) Europe PMC

SASDCR6 – Rap guanine nucleotide exchange factor 3 (isoform3) - binary form with cAMP

Rap guanine nucleotide exchange factor 3 (dimer)
MWI(0) 178 kDa
MWexpected 200 kDa
VPorod 415 nm3
log I(s) 1.97×100 1.97×10-1 1.97×10-2 1.97×10-3
Rap guanine nucleotide exchange factor 3 (dimer) small angle scattering data  s, nm-1
ln I(s)
Rap guanine nucleotide exchange factor 3 (dimer) Guinier plot ln 1.97×100 Rg: 5.3 nm 0 (5.3 nm)-2 s2
(sRg)2I(s)/I(0)
Rap guanine nucleotide exchange factor 3 (dimer) Kratky plot 1.104 0 3 sRg
p(r)
Rap guanine nucleotide exchange factor 3 (dimer) pair distance distribution function Rg: 5.2 nm 0 Dmax: 15.7 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 (dimer) CORAL model

log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 (dimer) DAMMIF model

SAXS data from solutions of Rap guanine nucleotide exchange factor 3 (isoform3) - binary form with cAMP - in 1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, 10mM Tris, pH 9 were collected using a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) equipped with a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). A sample concentration of 4 mg/ml was measured at 10°C. SAXS data were acquired from 4 successive 1800 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to generate the data displayed for this entry.

Rap guanine nucleotide exchange factor 3 (dimer) (EPAC1B (dimer))
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   100.0 kDa
 
UniProt   O95398-3 (2-881)
Sequence   FASTA
 
PDB code   4ZF7