Solution Structure Analysis Of An Exchange Protein Directly Activated By cAMP

Mark White.

SASDCS6 – Rap guanine nucleotide exchange factor 3 (isoform3) bound to RAS related protein 1b - with cAMP

Rap guanine nucleotide exchange factor 3
RAS related protein 1b
MWexperimental 119 kDa
MWexpected 118 kDa
VPorod 207 nm3
log I(s) 8.57×104 8.57×103 8.57×102 8.57×101
Rap guanine nucleotide exchange factor 3 RAS related protein 1b small angle scattering data  s, nm-1
ln I(s)
Rap guanine nucleotide exchange factor 3 RAS related protein 1b Guinier plot ln 8.58×104 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Rap guanine nucleotide exchange factor 3 RAS related protein 1b Kratky plot 1.104 0 3 sRg
p(r)
Rap guanine nucleotide exchange factor 3 RAS related protein 1b pair distance distribution function Rg: 4.3 nm 0 Dmax: 14.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 RAS related protein 1b SWISSMODEL model

log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 RAS related protein 1b DAMMIF model

SAXS data from solutions of Rap guanine nucleotide exchange factor 3 (isoform3) bound to RAS related protein 1b - with cAMP - in 1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, 10mM Tris, pH 9 were collected using a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) equipped with a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 4 mg/ml were measured at 10°C. SAXS data were acquired from 5 successive 1800 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration to generate the data displayed for this entry.

Rap guanine nucleotide exchange factor 3 (EPAC1B)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   100.0 kDa
 
UniProt   O95398-3 (2-881)
Sequence   FASTA
 
RAS related protein 1b (Rap1b)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   18 kDa
 
UniProt   q52l50 (1-167)
Sequence   FASTA
 
PDB code   3CF6