Conformational States of Exchange Protein Directly Activated by cAMP (EPAC1) Revealed by Ensemble Modeling and Integrative Structural Biology.

White MA, Tsalkova T, Mei FC, Cheng X, Cells 9(1) (2019) Europe PMC

SASDCS6 – Rap guanine nucleotide exchange factor 3 (isoform3) bound to RAS related protein 1b - with cAMP

Rap guanine nucleotide exchange factor 3
RAS related protein 1b
MWexperimental 119 kDa
MWexpected 118 kDa
VPorod 207 nm3
log I(s) 8.57×104 8.57×103 8.57×102 8.57×101
Rap guanine nucleotide exchange factor 3 RAS related protein 1b small angle scattering data  s, nm-1
ln I(s)
Rap guanine nucleotide exchange factor 3 RAS related protein 1b Guinier plot ln 8.58×104 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Rap guanine nucleotide exchange factor 3 RAS related protein 1b Kratky plot 1.104 0 3 sRg
p(r)
Rap guanine nucleotide exchange factor 3 RAS related protein 1b pair distance distribution function Rg: 4.3 nm 0 Dmax: 14.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 RAS related protein 1b SWISSMODEL model

log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 RAS related protein 1b DAMMIF model

SAXS data from solutions of Rap guanine nucleotide exchange factor 3 (isoform3) bound to RAS related protein 1b - with cAMP - in 1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, 10mM Tris, pH 9 were collected using a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) equipped with a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Solute concentrations ranging between 1 and 4 mg/ml were measured at 10°C. SAXS data were acquired from 5 successive 1800 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration to generate the data displayed for this entry.

Rap guanine nucleotide exchange factor 3 (EPAC1B)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   100.0 kDa
 
UniProt   O95398-3 (2-881)
Sequence   FASTA
 
RAS related protein 1b (Rap1b)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   18 kDa
 
UniProt   Q52L50 (1-167)
Sequence   FASTA
 
PDB code   3CF6