Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release.

Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC, Nat Commun 2017 Nov 22;8(1):1708 PubMed

SASDCY5 – Dimeric Sortilin at pH 5.5

Sortilin, also: Neurotensin-receptor 3
MWI(0) 168 kDa
MWexpected 153 kDa
VPorod 336 nm3
log I(s) 5.31×101 5.31×100 5.31×10-1 5.31×10-2
Sortilin, also: Neurotensin-receptor 3 small angle scattering data  s, nm-1
ln I(s)
Sortilin, also: Neurotensin-receptor 3 Guinier plot ln 5.32×101 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Sortilin, also: Neurotensin-receptor 3 Kratky plot 1.104 0 3 sRg
p(r)
Sortilin, also: Neurotensin-receptor 3 pair distance distribution function Rg: 3.8 nm 0 Dmax: 11 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Sortilin, also: Neurotensin-receptor 3 CORAL model
log I(s)
 s, nm-1
Sortilin, also: Neurotensin-receptor 3 DAMMIF model
Synchrotron SAXS data from solutions of dimeric sortilin at pH 5.5 in 25 mM MES pH 5.5, 150 mM NaCl, pH 5, were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Size-exclusion chromatography SAXS (SEC-SAXS) was performed at 20°C where 2600 successive 1 second frames were collected through the elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted from the SAXS data measured from the sample component elution peaks.

Sortilin, also: Neurotensin-receptor 3 (Sort1, also: NTR3)
Mol. type   Protein
Organism   Mus musculus
Olig. state   Dimer
Mon. MW   76.6 kDa
 
UniProt   Q6PHU5
Sequence   FASTA