Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research.

Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C, Structure 26(2):345-355.e5 (2018) Europe PMC

SASDCY6 – T2 in b-DDM micelle (SEC-SAXS)

Mechanosensitive channel T2
n-Dodecyl-β-D-Maltopyranoside
MWexperimental 355 kDa
MWexpected 307 kDa
VPorod 896 nm3
log I(s) 2.51×102 2.51×101 2.51×100 2.51×10-1
Mechanosensitive channel T2 n-Dodecyl-β-D-Maltopyranoside small angle scattering data  s, nm-1
ln I(s)
Mechanosensitive channel T2 n-Dodecyl-β-D-Maltopyranoside Guinier plot ln 2.51×102 Rg: 5.6 nm 0 (5.6 nm)-2 s2
(sRg)2I(s)/I(0)
Mechanosensitive channel T2 n-Dodecyl-β-D-Maltopyranoside Kratky plot 1.104 0 √3 sRg
p(r)
Mechanosensitive channel T2 n-Dodecyl-β-D-Maltopyranoside pair distance distribution function Rg: 5.6 nm 0 Dmax: 16.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Mechanosensitive channel T2 n-Dodecyl-β-D-Maltopyranoside DAMMIF model
Synchrotron SAXS data from solutions of T2 in b-DDM micelle (SEC-SAXS) in 20 mM Tris 150 mM NaCl 5 % glycerol 0.03 % DDM, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 3600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Concentration = UNKNOWN

Mechanosensitive channel T2 (T2)
Mol. type   Protein
Organism   Thermoplasma volcanium
Olig. state   Heptamer
Mon. MW   32.9 kDa
 
UniProt   Q97AH5 (1-197)
Sequence   FASTA
 
n-Dodecyl-β-D-Maltopyranoside (b-DDM)
Mol. type   Other
Organism   synthetic construct
Olig. state   Other
Mon. MW   0.5 kDa