Structure and dynamics of the RNAPII CTDsome with Rtt103

Jasnovidova O, Klumpler T, Kubicek K, Kalynych S, Plevka P, Stefl R, Proceedings of the National Academy of Sciences 2017 Oct 4 :201712450 DOI

SASDCZ2 – Yeast CTDsome: transcription termination factor Rtt103p/C-terminal domain of DNA-directed RNA polymerase II subunit RPB1 (Rtt103 1-246-phospho-mimetic complex)

Regulator of Ty1 transposition protein 103
DNA-directed RNA polymerase II subunit RPB1
MWexperimental 184.5 kDa
MWexpected 70 kDa
VPorod 561 nm3
log I(s) 1.80×10-1 1.80×10-2 1.80×10-3 1.80×10-4
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 small angle scattering data  s, nm-1
ln I(s)
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 Guinier plot ln 1.80×10-1 Rg: 5.3 nm 0 (5.3 nm)-2 s2
(sRg)2I(s)/I(0)
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 Kratky plot 1.104 0 3 sRg
p(r)
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 pair distance distribution function Rg: 5.8 nm 0 Dmax: 21 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 CORAL model
log I(s)
 s, nm-1
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 CORAL model
log I(s)
 s, nm-1
Regulator of Ty1 transposition protein 103 DNA-directed RNA polymerase II subunit RPB1 CORAL model
SAXS data from solutions of Rtt103 1-246-phospho-mimetic complex in 25 mM KH2PO4, 300 mM KCl, 10 mM β-mercaptoethanol, pH 6.5 were collected using a BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Five successive 3600 second frames were collected at a sample temperature of 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged and the corresponding scattering from the solvent-blank was subtracted to produce the scattering profile displayed in this entry.

Regulator of Ty1 transposition protein 103
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Dimer
Mon. MW   28.6 kDa
 
UniProt   Q05543
Sequence   FASTA
 
DNA-directed RNA polymerase II subunit RPB1
Mol. type   Protein
Organism   Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Olig. state   Monomer
Mon. MW   12.8 kDa
 
UniProt   P04050
Sequence   FASTA
 
PDB code   5M48
 
PDB code   5M48