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8 hits found for Klumpler

SASDC62 – Basic domain of telomeric repeat-binding factor 2 (TRF2)

Basic domain of telomeric repeat-binding factor 2 experimental SAS data
Basic domain of telomeric repeat-binding factor 2 Kratky plot
Sample: Basic domain of telomeric repeat-binding factor 2 monomer, 5 kDa Homo sapiens protein
Buffer: 50 mM NaPi, 50 mM NaCl, pH: 7
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2015 Mar 5
Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
...Klumpler T, Hofr C
RgGuinier 1.7 nm
Dmax 7.1 nm
VolumePorod 4 nm3

SASDCZ2 – Yeast CTDsome: transcription termination factor Rtt103p/C-terminal domain of DNA-directed RNA polymerase II subunit RPB1 (Rtt103 1-246-phospho-mimetic complex)

Regulator of Ty1 transposition protein 103DNA-directed RNA polymerase II subunit RPB1 experimental SAS data
CORAL model
Sample: Regulator of Ty1 transposition protein 103 dimer, 57 kDa Saccharomyces cerevisiae (strain … protein
DNA-directed RNA polymerase II subunit RPB1 monomer, 13 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM KH2PO4, 300 mM KCl, 10 mM BME, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 Apr 12
Structure and dynamics of the RNAPII CTDsome with Rtt103. Proc Natl Acad Sci U S A 114(42):11133-11138 (2017)
...Klumpler T, Kubicek K, Kalynych S, Plevka P, Stefl R
RgGuinier 5.3 nm
Dmax 21.0 nm
VolumePorod 561 nm3

SASDDN6 – Human stress-inducible heat shock 70 kDa protein 1, Hsp70 (Hspa1a) T204A mutant dimer, in the presence of ATP

Heat shock 70 kDa protein 1 experimental SAS data
MODELLER model
Sample: Heat shock 70 kDa protein 1 dimer, 147 kDa Homo sapiens protein
Buffer: 50mM HEPES, 150mM KCH3COO, 2mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2018 Feb 20
Human stress-inducible Hsp70 has a high propensity to form ATP-dependent antiparallel dimers that are differentially regulated by co-chaperone binding. Mol Cell Proteomics (2018)
...Klumpler T, Vojtesek B, Muller P, Man P
RgGuinier 4.0 nm
Dmax 11.6 nm
VolumePorod 248 nm3

SASDF29 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4ADP-ribosylation factor1 - short experimental SAS data
OTHER model
Sample: Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 monomer, 8 kDa Homo sapiens protein
ADP-ribosylation factor1 - short monomer, 11 kDa Homo sapiens RNA
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH: 6.8
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2015 Dec 10
Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition. Nucleic Acids Res (2019)
...Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ
RgGuinier 1.7 nm
Dmax 6.0 nm
VolumePorod 21 nm3

SASDF39 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1

ADP-ribosylation factor1 - shortDouble-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 experimental SAS data
OTHER model
Sample: ADP-ribosylation factor1 - short monomer, 11 kDa Homo sapiens RNA
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 monomer, 20 kDa Homo sapiens protein
Buffer: 50 mM sodium phosphate buffer, 300 mM NaCl, 500 mM imidazole, pH: 8
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2018 May 29
Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition. Nucleic Acids Res (2019)
...Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ
RgGuinier 2.1 nm
Dmax 6.4 nm
VolumePorod 39 nm3

SASDF49 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4ADP-ribosylation factor1 - long experimental SAS data
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long Kratky plot
Sample: Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 monomer, 20 kDa Homo sapiens protein
ADP-ribosylation factor1 - long monomer, 16 kDa Homo sapiens RNA
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH: 6.8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 20
Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition. Nucleic Acids Res (2019)
...Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ
RgGuinier 2.5 nm
Dmax 7.0 nm
VolumePorod 53 nm3

SASDB79 – Basic domain of human telomeric repeat-binding factor 2 (TRF2) in complex with telomeric DNA duplex

Basic domain of telomeric repeat-binding factor 2telomere DNA duplex experimental SAS data
MONSA model
Sample: Basic domain of telomeric repeat-binding factor 2 monomer, 5 kDa Homo sapiens protein
telomere DNA duplex monomer, 11 kDa DNA
Buffer: 20 mM Tris-HCl, 50 mM LiCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 May 3
Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
...Klumpler T, Hofr C
RgGuinier 1.7 nm
Dmax 6.0 nm
VolumePorod 20 nm3

SASDB89 – Telomeric DNA duplex

telomere DNA duplex experimental SAS data
MONSA model
Sample: telomere DNA duplex monomer, 11 kDa DNA
Buffer: 20 mM Tris-HCl, 50 mM LiCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 May 3
Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
...Klumpler T, Hofr C
RgGuinier 1.6 nm
Dmax 5.7 nm
VolumePorod 12 nm3