Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release.

Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC, Nat Commun 8(1):1708 (2017) Europe PMC

SASDCZ5 – Monomeric Sortilin at pH 7.4

Sortilin 1 A464E alias Neurotensin-receptor 3 A464E
MWI(0) 86 kDa
MWexpected 76 kDa
VPorod 217 nm3
log I(s) 2.51×101 2.51×100 2.51×10-1 2.51×10-2
Sortilin 1 A464E alias Neurotensin-receptor 3 A464E small angle scattering data  s, nm-1
ln I(s)
Sortilin 1 A464E alias Neurotensin-receptor 3 A464E Guinier plot ln 2.51×101 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Sortilin 1 A464E alias Neurotensin-receptor 3 A464E Kratky plot 1.104 0 3 sRg
p(r)
Sortilin 1 A464E alias Neurotensin-receptor 3 A464E pair distance distribution function Rg: 3.3 nm 0 Dmax: 10.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Sortilin 1 A464E alias Neurotensin-receptor 3 A464E CORAL model

log I(s)
 s, nm-1
Sortilin 1 A464E alias Neurotensin-receptor 3 A464E DAMMIF model

Synchrotron SAXS data from solutions of monomeric sortilin at pH 7.4 in 25 mM HEPES, 150 mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Size-exclusion chromatography SAXS (SEC-SAXS) was performed at 20°C where 2600 successive 1 second frames were collected through the elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted from the SAXS data measured from the sample component elution peaks.

Sortilin 1 A464E alias Neurotensin-receptor 3 A464E
Mol. type   Protein
Organism   Mus musculus
Olig. state   Monomer
Mon. MW   76.5 kDa
 
UniProt   Q6PHU5
Sequence   FASTA