Dmax unknown – experimental data range validation not possible.
There are no models related to this curve.
Synchrotron SAXS data from solutions of Candida antarctica lipase B - with a guanidine-HCl unfolding series under reducing conditions - in 100 mM NaCl, 20 mM Na2HPO4, 10 mM DTT, pH 6, were collected on the EMBL-P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.65 mg/ml was measured at 10°C. 29 successive 0.030 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
In addition to the SAXS profile displayed above, two lipase B unfolding series in the presence of DTT spanning 0 M to 6 M guanidine hydrochloride are included in the full entry zip archive.