Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions.

Franke D, Jeffries CM, Svergun DI
Biophys J 114(11):2485-2492 (2018 Jun 5)

PMID: 29874600 doi: 10.1016/j.bpj.2018.04.018

Published in SASBDB: 2018 Jun 7

SASDDJ3 – Candida antarctica lipase B - with guanidine-HCl unfolding series Sample: Lipase B from Pseudozyma antarctica, 33 kDa Moesziomyces antarcticus protein Buffer: 100 mM NaCl, 20 mM Na2HPO4, pH: 6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29 RgGuinier 2.4 nm

SASDDK3 – Candida antarctica lipase B - with guanidine-HCl unfolding series, in the presence of dithiothreitol Sample: Lipase B from Pseudozyma antarctica, 33 kDa Moesziomyces antarcticus protein Buffer: 100 mM NaCl, 20 mM Na2HPO4, 10 mM DTT, pH: 6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29 RgGuinier 2.4 nm

SASDDL3 – Folded ribonuclease A (RNAse) Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein Buffer: phosphate buffered saline (PBS), pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29 RgGuinier 1.6 nm Dmax 5.6 nm VolumePorod 16 nm3

SASDDM3 – Carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea Sample: Ribonuclease pancreatic monomer, 16 kDa Bos taurus protein Buffer: 10 mM HCl, pH: 1 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 29 RgGuinier 2.3 nm Dmax 9.0 nm

SASDDN3 – Bovine serum albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets) Sample: Bovine serum albumin, 66 kDa Bos taurus protein Buffer: 50 mM HEPES, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 25 RgGuinier 3.0 nm Dmax 11.0 nm VolumePorod 117 nm3