Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function.

Luo M, Gamage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ, J Biol Chem 291(46):24065-24075 (2016) PubMed

SASDDL2 – Sinorhizobium meliloti Proline Utilization A (PutA) lowest concentration, 1.00 mg/ml

Sinorhizobium meliloti (SmPutA)
MWexperimental 130 kDa
MWexpected 132 kDa
VPorod 171 nm3
log I(s) 7.69×102 7.69×101 7.69×100 7.69×10-1
Sinorhizobium meliloti (SmPutA) small angle scattering data  s, nm-1
ln I(s)
Sinorhizobium meliloti (SmPutA) Guinier plot ln 7.69×102 Rg: 3.4 nm 0 (3.4 nm)-2 s2
Sinorhizobium meliloti (SmPutA) Kratky plot 1.104 0 3 sRg
Sinorhizobium meliloti (SmPutA) pair distance distribution function Rg: 3.5 nm 0 Dmax: 11.0 nm

Experimental data validation

Fits and models

log I(s)
 s, nm-1
Sinorhizobium meliloti (SmPutA) MES-FOXS model
Synchrotron SAXS data from solutions of Sinorhizobium meliloti proline utilization A (PutA) in 50 mM Tris, 1% (v/v) glycerol, 0.5 mM THP and 50 mM NaCl, pH 7.8, were collected on the 12.3.1 SIBYLS beam line at the Advanced Light Source storage ring (ALS; Berkeley, CA, USA) using a MAR 165 CCD detector at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sinorhizobium meliloti (SmPutA) (SmPutA)
Mol. type   Protein
Organism   Sinorhizobium meliloti
Olig. state   Monomer
Mon. MW   131.8 kDa
UniProt   F7X6I3
Sequence   FASTA