Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions.

Franke D, Jeffries CM, Svergun DI, Biophys J 114(11):2485-2492 (2018) Europe PMC

SASDDM3 – Carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea

Ribonuclease pancreatic
MWI(0) 13 kDa
MWexpected 16 kDa
log I(s) 4.84×102 4.84×101 4.84×100 4.84×10-1
Ribonuclease pancreatic small angle scattering data  s, nm-1
ln I(s)
Ribonuclease pancreatic Guinier plot ln 4.85×102 Rg: 2.3 nm 0 (2.3 nm)-2 s2
Ribonuclease pancreatic Kratky plot 1.104 0 3 sRg
Ribonuclease pancreatic pair distance distribution function Rg: 2.6 nm 0 Dmax: 9 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of carboyxamidomethylated ribonuclease A (unfolded RNAse) - with and without urea in 10 mM HCl, pH 1, were collected on the EMBL-P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.97 mg/ml was measured at 10°C. 17 successive 0.030 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Included in the additional files of the full-entry zip archive are SAXS data measured from folded RNAse (rnase.dat - also refer to SASBDB entry SASDDL3) as well as unfolded carboyxamidomethylated ribonuclease A (rnaseU.dat) and unfolded RNAse in the presence of 1 M urea (rnaseUU1.dat) or 2 M urea (rnaseUU2.dat).

Ribonuclease pancreatic (RNase)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Monomer
Mon. MW   16.5 kDa
UniProt   P61823
Sequence   FASTA