Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions.

Franke D, Jeffries CM, Svergun DI, Biophys J 114(11):2485-2492 (2018) Europe PMC

SASDDN3 – Bovine serum albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets)

Bovine serum albumin
MWexperimental 72 kDa
MWexpected 66 kDa
VPorod 117 nm3
log I(s) 1.15×104 1.15×103 1.15×102 1.15×101
Bovine serum albumin small angle scattering data  s, nm-1
ln I(s)
Bovine serum albumin Guinier plot ln 1.16×104 Rg: 3.0 nm 0 (3.0 nm)-2 s2
Bovine serum albumin Kratky plot 1.104 0 3 sRg
Bovine serum albumin pair distance distribution function Rg: 3.1 nm 0 Dmax: 11 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from a solution of bovine serum albumin (mixture) in 50 mM HEPES, pH 7.5, were collected on the EMBL-P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.25 mg/ml was measured at 10°C. 100 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The data displayed in this entry represents the averaged 1D-SAXS profile obtained from 100 individual SAXS data frames. Included in the full entry zip-archive are each of the individual subtracted SAXS profiles as well as each individual sample and buffer frame (100 in total).

Bovine serum albumin (BSA)
Mol. type   Protein
Organism   Bos taurus
Olig. state   Unknown
Mon. MW   66.5 kDa
UniProt   P02769 (25-607)
Sequence   FASTA