The dynamic nature of the conserved tegument protein UL37 of herpesviruses.

Koenigsberg A, Heldwein E, Journal of Biological Chemistry :jbc.RA118.004481 (2018) DOI

SASDDP9 – C-terminal half of pseudorabies virus tegument protein UL37

Tegument protein UL37
MWexperimental 48 kDa
MWexpected 49 kDa
VPorod 71 nm3
log I(s) 1.74×10-2 1.74×10-3 1.74×10-4 1.74×10-5
Tegument protein UL37 small angle scattering data  s, nm-1
ln I(s)
Tegument protein UL37 Guinier plot ln 1.75×10-2 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
Tegument protein UL37 Kratky plot 1.104 0 3 sRg
p(r)
Tegument protein UL37 pair distance distribution function Rg: 4.3 nm 0 Dmax: 14 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Tegument protein UL37 DAMMIN model
Tegument protein UL37 DAMFILT model

Synchrotron SAXS data, I(s) vs s (where s = 4πsinθ/λ, and 2θ is the scattering angle), were measured from the C-terminal half of the Pseudorabiesvirus tegument protein UL37 using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA). Data were collected a using a Pilatus 200K detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.125 nm. The SEC mobile phase consisted of 100 mM HEPES, 150 mM NaCl, 5% glycerol, 0.1 mM tris(2-carboxyethyl)phosphine (TCEP), pH 7.5, (4°C). The SAXS data measured from the SEC-elution (sample peak and buffer; 300 successive 2 s frames) were normalized to the intensity of the transmitted beam and radially averaged. The scattering of an appropriate solvent-blank was subtracted from the sample frames to produce the scaled and averaged data displayed in this entry.

Additional SEC parameters: Column type: Superdex 200 Increase 5/150 GE; Flow rate: 0.3 ml/min; Sample injection concentration: 6 mg/ml; Injection volume: 100 µl. The models displayed in this entry are: Top, individual best-fit DAMMIN model and; Bottom, the bead-occupancy and volume-corrected averaged spatial representation of the protein (DAMFILT model) obtained from ten individual DAMMIN runs (NSD = 0.78; resolution estimate = 4.1 nm).

Tegument protein UL37
Mol. type   Protein
Organism   Suid alphaherpesvirus 1
Olig. state   Monomer
Mon. MW   48.5 kDa
 
UniProt   Q911W0 (478-919)
Sequence   FASTA