The dynamic nature of the conserved tegument protein UL37 of herpesviruses.

Koenigsberg AL, Heldwein EE, J Biol Chem 293(41):15827-15839 (2018) Europe PMC

SASDDQ9 – N-terminal half of herpes simplex virus type-1 inner tegument protein UL37

Inner tegument protein
MWexperimental 63 kDa
MWexpected 62 kDa
VPorod 91 nm3
log I(s) 2.78×10-2 2.78×10-3 2.78×10-4 2.78×10-5
Inner tegument protein small angle scattering data  s, nm-1
ln I(s)
Inner tegument protein Guinier plot ln 2.79×10-2 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Inner tegument protein Kratky plot 1.104 0 √3 sRg
p(r)
Inner tegument protein pair distance distribution function Rg: 3.4 nm 0 Dmax: 10.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Inner tegument protein DAMFILT model
Inner tegument protein DAMMIN model
Synchrotron SAXS data, I(s) vs s (where s = 4πsinθ/λ, and 2θ is the scattering angle), were measured from the N-terminal half of herpes simplex virus type-1 inner tegument protein UL37 using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA). Data were collected a using a Pilatus 200K detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.125 nm. The SEC mobile phase consisted of 100 mM HEPES, 150 mM NaCl, 5% glycerol ,0.1 mM tris(2-carboxyethyl)phosphine (TCEP), pH 7.5, (4°C). The SAXS data measured from the SEC-elution (sample peak and buffer; 300 successive 2 s frames) were normalized to the intensity of the transmitted beam and radially averaged. The scattering of an appropriate solvent-blank was subtracted from the sample frames to produce the scaled and averaged data displayed in this entry.

Additional SEC parameters: Column type: Superdex 200 Increase 5/150 GE; Flow rate: 0.3 mL/min; Sample injection concentration: 6 mg/ml; Injection volume: 100 µl.

Inner tegument protein (HSV1 UL37)
Mol. type   Protein
Organism   Human alphaherpesvirus 1
Olig. state   Monomer
Mon. MW   61.7 kDa
 
UniProt   P10221
Sequence   FASTA