Synchrotron SAXS data from solutions of Yeast tRNA Nm34 methyltransferase Trm7-Trm734 complex in 50 mM HEPES-KOH, 200 mM, KCl, 5 % v/v glycerol, 10 mM β-mercaptoethanol, pH 8.0 were collected on the beam line BL-10C at the Phton Factory (PF; Tsukuba, Ibaraki, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.05 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). 40 images of 20 second frames were collected at 20°C. The data were normalized to the intensity of the incident beam and circularly averaged; the scattering of the solvent-blank was subtracted. The extrapolated scattering profile at a concentration of zero was calculated PRIMUS QT of ATSAS.
|
Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
(Trm7_yeast)
|
Mol. type |
|
Protein |
Organism |
|
Saccharomyces cerevisiae |
Olig. state |
|
Monomer |
Mon. MW |
|
36.5 kDa |
|
UniProt |
|
P38238
(1-310)
|
Sequence |
|
FASTA |
|
Trm734: Regulator of Ty1 transposition protein 10
(Trm734 (WDR6_yeast))
|
Mol. type |
|
Protein |
Organism |
|
Saccharomyces cerevisiae |
Olig. state |
|
Monomer |
Mon. MW |
|
116.3 kDa |
|
UniProt |
|
Q08924
(1-1013)
|
Sequence |
|
FASTA |
|
|