Structure of tRNA methyltransferase complex of Trm7 and Trm734 reveals a novel binding interface for tRNA recognition.

Hirata A, Okada K, Yoshii K, Shiraishi H, Saijo S, Yonezawa K, Shimizu N, Hori H, Nucleic Acids Res (2019) Europe PMC

SASDDR3 – Yeast tRNA Nm34 methyltransferase Trm7-Trm734 complex from Sacharomyces cerevisiae

Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
Trm734: Regulator of Ty1 transposition protein 10
MWexperimental 159 kDa
MWexpected 153 kDa
VPorod 218 nm3
log I(s) 1.92×10-1 1.92×10-2 1.92×10-3 1.92×10-4
Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase Trm734: Regulator of Ty1 transposition protein 10 small angle scattering data  s, nm-1
ln I(s)
Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase Trm734: Regulator of Ty1 transposition protein 10 Guinier plot ln 1.93×10-1 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase Trm734: Regulator of Ty1 transposition protein 10 Kratky plot 1.104 0 √3 sRg
p(r)
Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase Trm734: Regulator of Ty1 transposition protein 10 pair distance distribution function Rg: 3.8 nm 0 Dmax: 13.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase Trm734: Regulator of Ty1 transposition protein 10 CORAL model
log I(s)
 s, nm-1
Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase Trm734: Regulator of Ty1 transposition protein 10 DAMMIF model
Synchrotron SAXS data from solutions of Yeast tRNA Nm34 methyltransferase Trm7-Trm734 complex in 50 mM HEPES-KOH, 200 mM, KCl, 5 % v/v glycerol, 10 mM β-mercaptoethanol, pH 8.0 were collected on the beam line BL-10C at the Phton Factory (PF; Tsukuba, Ibaraki, Japan) using a Pilatus3 2M detector at a sample-detector distance of 2.05 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). 40 images of 20 second frames were collected at 20°C. The data were normalized to the intensity of the incident beam and circularly averaged; the scattering of the solvent-blank was subtracted. The extrapolated scattering profile at a concentration of zero was calculated PRIMUS QT of ATSAS.

Trm7: tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase (Trm7_yeast)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Monomer
Mon. MW   36.5 kDa
 
UniProt   P38238 (1-310)
Sequence   FASTA
 
Trm734: Regulator of Ty1 transposition protein 10 (Trm734 (WDR6_yeast))
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Monomer
Mon. MW   116.3 kDa
 
UniProt   Q08924 (1-1013)
Sequence   FASTA