A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions.

Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J, ACS Comb Sci 20(4):197-202 (2018) PubMed

SASDDY4 – Protein sex-lethal mutant with 10GS-linker

Protein sex-lethal mutant
MWexperimental 16 kDa
MWexpected 20 kDa
VPorod 27 nm3
log I(s) 2.26×101 2.26×100 2.26×10-1 2.26×10-2
Protein sex-lethal mutant small angle scattering data  s, nm-1
ln I(s)
Protein sex-lethal mutant Guinier plot ln 2.27×101 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
Protein sex-lethal mutant Kratky plot 1.104 0 3 sRg
p(r)
Protein sex-lethal mutant pair distance distribution function Rg: 2.2 nm 0 Dmax: 7.4 nm

Experimental data validation


Fits and models


log I(s)
 s, nm-1
Protein sex-lethal mutant with 10GS-linker Rg histogram Rg, nm
Protein sex-lethal mutant EOM/RANCH model
Protein sex-lethal mutant EOM/RANCH model
Protein sex-lethal mutant EOM/RANCH model
Protein sex-lethal mutant EOM/RANCH model
Protein sex-lethal mutant EOM/RANCH model
Synchrotron SAXS data from solutions of Protein sex-lethal mutant with 10GS-linker in 10 mM KP, 50 mM NaCl, 10 mM DTT, pH 6 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Protein sex-lethal mutant (Sxl10GS)
Mol. type   Protein
Organism   Drosophila melanogaster
Olig. state   Monomer
Mon. MW   20.4 kDa
 
UniProt   P19339 (123-294)
Sequence   FASTA