A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions.

Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
ACS Comb Sci 20(4):197-202 (2018 Apr 9)
PMID: 29553252
doi: 10.1021/acscombsci.8b00007
Submitted to SASBDB: 2018 Mar 15
Published in SASBDB:

SASDDX4 – Wild-type Protein sex-lethal

Protein sex-lethal experimental SAS data
Protein sex-lethal Kratky plot
Sample: Protein sex-lethalmonomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 16
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 26 nm3

SASDDY4 – Protein sex-lethal mutant with 10GS-linker

Protein sex-lethal mutant experimental SAS data
Protein sex-lethal mutant with 10GS-linker Rg histogram
Sample: Protein sex-lethal mutantmonomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 2
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 27 nm3

SASDDZ4 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU

Protein sex-lethal mutantRNA decaneucleotide U8GU experimental SAS data
1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU Rg histogram
Sample: Protein sex-lethal mutantmonomer, 20 kDa Drosophila melanogaster protein
RNA decaneucleotide U8GUmonomer, 3 kDa synthetic construct RNA
Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 3
RgGuinier 2.2 nm
Dmax 7.8 nm
VolumePorod 34 nm3

SASDD25 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8

Protein sex-lethal mutantRNA decaneucleotide UGU8 experimental SAS data
1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8 Rg histogram
Sample: Protein sex-lethal mutantdimer, 41 kDa Drosophila melanogaster protein
RNA decaneucleotide UGU8dimer, 6 kDa synthetic construct RNA
Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 12
RgGuinier 2.5 nm
Dmax 8.9 nm
VolumePorod 41 nm3