A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.

Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R, Nat Commun 10(1):972 (2019) Europe PMC

SASDE48 – Toxin GraT

Putative killer protein, Toxin GraT.
MWexperimental 11 kDa
MWexpected 11 kDa
VPorod 18 nm3
log I(s) 1.42×10-2 1.42×10-3 1.42×10-4 1.42×10-5
Putative killer protein, Toxin GraT. small angle scattering data  s, nm-1
ln I(s)
Putative killer protein, Toxin GraT. Guinier plot ln 1.42×10-2 Rg: 1.5 nm 0 (1.5 nm)-2 s2
(sRg)2I(s)/I(0)
Putative killer protein, Toxin GraT. Kratky plot 1.104 0 √3 sRg
p(r)
Putative killer protein, Toxin GraT. pair distance distribution function Rg: 1.6 nm 0 Dmax: 5.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Toxin GraT in 50 mM Tris 250 mM NaCl 2 mM TCEP, pH 8 were collected using using size exclusion chromatography SAXS (SEC-SAXS) on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103317 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 25°C using the following parameters: Column: Shodex KW402.5-4F; Flow rate: 0.2 mL/min; Total acquisition time: 17 min; Sample injection concentration: 12.5 mg/mL; Injection volume: 40 μL. 40 successive 0.990 second frames were collected through the SEC elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Putative killer protein, Toxin GraT. (GraT)
Mol. type   Protein
Organism   Pseudomonas putida
Olig. state   Monomer
Mon. MW   11.1 kDa
 
UniProt   Q88MI5 (1-91)
Sequence   FASTA