A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.

Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R, Nat Commun 10(1):972 (2019) Europe PMC

SASDE48 – Toxin GraT

Putative killer protein, Toxin GraT.
MWexperimental 11 kDa
MWexpected 11 kDa
VPorod 18 nm3
log I(s) 1.42×10-2 1.42×10-3 1.42×10-4 1.42×10-5
Putative killer protein, Toxin GraT. small angle scattering data  s, nm-1
ln I(s)
Putative killer protein, Toxin GraT. Guinier plot ln 1.42×10-2 Rg: 1.5 nm 0 (1.5 nm)-2 s2
(sRg)2I(s)/I(0)
Putative killer protein, Toxin GraT. Kratky plot 1.104 0 3 sRg
p(r)
Putative killer protein, Toxin GraT. pair distance distribution function Rg: 1.6 nm 0 Dmax: 5.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Toxin GraT in 50 mM Tris 250 mM NaCl 2 mM TCEP, pH 8 were collected using using size exclusion chromatography SAXS (SEC-SAXS) on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103317 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 25°C using the following parameters: Column: Shodex KW402.5-4F; Flow rate: 0.2 mL/min; Total acquisition time: 17 min; Sample injection concentration: 12.5 mg/mL; Injection volume: 40 μL. 40 successive 0.990 second frames were collected through the SEC elution profile. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

Putative killer protein, Toxin GraT. (GraT)
Mol. type   Protein
Organism   Pseudomonas putida
Olig. state   Monomer
Mon. MW   11.1 kDa
 
UniProt   Q88MI5 (1-91)
Sequence   FASTA