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3 hits found for Talavera

SASDE48 – Toxin GraT

Putative killer protein, Toxin GraT. experimental SAS data
Putative killer protein, Toxin GraT. Kratky plot
Sample: Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein
Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Sep 21
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
RgGuinier 1.5 nm
Dmax 5.5 nm
VolumePorod 18 nm3

SASDE58 – Complex of toxin GraT with antotoxin GraA

Putative killer protein, Toxin GraT.antitoxin GraA, antidote of toxin GraTPutative killer protein, Toxin GraT experimental SAS data
Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT Kratky plot
Sample: Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein
antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein
Putative killer protein, Toxin GraT monomer, 11 kDa Pseudomonas putida protein
Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jun 29
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
RgGuinier 2.3 nm
Dmax 7.9 nm
VolumePorod 63 nm3

SASDE68 – Two dimers of antitoxin GraA in complex with the grta operator

GraTA operator regionantitoxin GraA, antidote of toxin GraTatitoxin GraA, antidote of GraT. experimental SAS data
GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. Kratky plot
Sample: GraTA operator region monomer, 20 kDa DNA
antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein
atitoxin GraA, antidote of GraT. dimer, 23 kDa Pseudomonas putida protein
Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Dec 17
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
RgGuinier 2.9 nm
Dmax 10.3 nm
VolumePorod 94 nm3