A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.

Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R, Nat Commun 10(1):972 (2019) Europe PMC

SASDE58 – Complex of toxin GraT with antotoxin GraA

Putative killer protein, Toxin GraT.
antitoxin GraA, antidote of toxin GraT
Putative killer protein, Toxin GraT
MWI(0) 41 kDa
MWexpected 44 kDa
VPorod 63 nm3
log I(s) 4.88×10-2 4.88×10-3 4.88×10-4 4.88×10-5
Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT small angle scattering data  s, nm-1
ln I(s)
Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT Guinier plot ln 4.89×10-2 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT Kratky plot 1.104 0 3 sRg
p(r)
Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT pair distance distribution function Rg: 2.3 nm 0 Dmax: 7.9 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the complex formed between toxin GraT with antotoxin GraA in 50 mM Tris 250 mM NaCl 2 mM TCEP, pH 8 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.10332 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 10°C using the following parameters: Column: Shodex KW402.5-4F; Flow rate: 0.2 mL/min; Sample injection concentration: 12.5 mg/mL; Injection volume: 40 μL. 1200 successive 0.500 second frames were collected. The data measured through the SEC elution profile of the sample were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

Putative killer protein, Toxin GraT. (GraT)
Mol. type   Protein
Organism   Pseudomonas putida
Olig. state   Monomer
Mon. MW   11.1 kDa
 
UniProt   Q88MI5 (1-91)
Sequence   FASTA
 
antitoxin GraA, antidote of toxin GraT (GraA)
Mol. type   Protein
Organism   Pseudomonas putida
Olig. state   Dimer
Mon. MW   10.9 kDa
 
UniProt   Q88MI6 (1-99)
Sequence   FASTA
 
Putative killer protein, Toxin GraT (GraT.)
Mol. type   Protein
Organism   Pseudomonas putida
Olig. state   Monomer
Mon. MW   11.1 kDa
Sequence   FASTA