A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.

Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R, Nat Commun 10(1):972 (2019) Europe PMC

SASDE68 – Two dimers of antitoxin GraA in complex with the grta operator

GraTA operator region
antitoxin GraA, antidote of toxin GraT
atitoxin GraA, antidote of GraT.
MWI(0) 67 kDa
MWexpected 66 kDa
VPorod 94 nm3
log I(s) 1.71×10-2 1.71×10-3 1.71×10-4 1.71×10-5
GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. small angle scattering data  s, nm-1
ln I(s)
GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. Guinier plot ln 1.71×10-2 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. Kratky plot 1.104 0 3 sRg
p(r)
GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.3 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of antitoxin GraA in complex with the grta operator in 50 mM Tris 250 mM NaCl 2 mM TCEP, pH 8 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the SWING beam line at SOLEIL (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.10332 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 10°C using the following parameters: Column: Shodex KW402.5-4F; Flow rate: 0.2 mL/min; Sample injection concentration: approx. 10 mg/mL; Injection volume: 40 μL. 254 successive 1.500 second frames were collected. The SAXS data measured through the SEC elution profile of the sample were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

GraTA operator region
Mol. type   DNA
Olig. state   Monomer
Mon. MW   20.4 kDa
Sequence   FASTA
 
antitoxin GraA, antidote of toxin GraT (GraA)
Mol. type   Protein
Organism   Pseudomonas putida
Olig. state   Dimer
Mon. MW   10.9 kDa
 
UniProt   Q88MI6 (1-99)
Sequence   FASTA
 
atitoxin GraA, antidote of GraT. (GraA)
Mol. type   Protein
Organism   Pseudomonas putida
Olig. state   Dimer
Mon. MW   11.7 kDa
 
UniProt   Q88MI6 (1-99)
Sequence   FASTA