Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes.

Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA, Angew Chem Int Ed Engl (2019) Europe PMC

SASDE74 – Tryparedoxin W70A, oxidized state

Tryparedoxin W70A
MWexperimental 18 kDa
MWexpected 16 kDa
VPorod 31 nm3
log I(s) 1.13×101 1.13×100 1.13×10-1 1.13×10-2
Tryparedoxin W70A small angle scattering data  s, nm-1
ln I(s)
Tryparedoxin W70A Guinier plot ln 1.13×101 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
Tryparedoxin W70A Kratky plot 1.104 0 3 sRg
p(r)
Tryparedoxin W70A pair distance distribution function Rg: 1.8 nm 0 Dmax: 7.7 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Tryparedoxin W70A PDB model
Tryparedoxin W70A OTHER model

Synchrotron SAXS data from solutions of Tryparedoxin W70A, oxidized state in 10 mM HEPES pH 7.5, 50 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were collected using in-line size exclusion chromatography (SEC)-SAXS at 20°C.

SEC column type: GE Healthcare S75 3.2/300; Sample Injection Concentration = 10 mg/mL; Column flow-rate = 0.1 mL/min.

Tryparedoxin W70A
Mol. type   Protein
Organism   Trypanosoma brucei brucei
Olig. state   Monomer
Mon. MW   15.7 kDa
 
UniProt   O77404 (2-144(W70A))
Sequence   FASTA
 
PDB code   6GXY