SAXS data from solutions of Bovine serum albumin from in-house SAXS in 50 mM HEPES, pH 7.5 were collected on a Xenocs BioXolver L instrument at the Copenhagen University, Department of Drug Design and Pharmacology (Copenhagen, Denmark) using a 20Hz Pilatus 300K detector at a sample-detector distance of 0.7 m and at a wavelength of λ = 0.134 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.60 mg/ml was measured at 25°C. One 60 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The background corrected data was binned logarithmically, normalized by concentration and brought to absolute scale using water as a secondary standard.
|
Bovine serum albumin
(BSA)
|
Mol. type |
|
Protein |
Organism |
|
Bos taurus |
Olig. state |
|
Monomer |
Mon. MW |
|
66.4 kDa |
|
UniProt |
|
P02769
(25-607)
|
Sequence |
|
FASTA |
|
PDB code |
|
4F5S |
|
|