Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument

Bucciarelli S, Midtgaard S, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
Journal of Applied Crystallography 51(6) (2018 Dec 1)

doi: 10.1107/S1600576718014462
Submitted to SASBDB: 2018 Sep 29
Published in SASBDB:

SASDEY4 – Ribonuclease A monomer from in-house SEC-SAXS

Ribonuclease pancreatic experimental SAS data
PDB model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 10 nm3

SASDEZ4 – Carbonic Anhydrase from in-house SEC-SAXS

Carbonic anhydrase 2 experimental SAS data
PDB model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 24
RgGuinier 1.9 nm
Dmax 5.3 nm
VolumePorod 38 nm3

SASDE25 – Human insulin hexamer from in-house SEC-SAXS

Human insulin experimental SAS data
DAMFILT model
Sample: Human insulin hexamer, 35 kDa Homo sapiens protein
Buffer: 7 mM sodium phosphate, 60 mM phenol, 200 ┬ÁM Zn(CH3COO)2, 23 mM NaCl, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 26
RgGuinier 2.0 nm
Dmax 5.9 nm
VolumePorod 39 nm3

SASDE35 – Ovalbumin monomer from in-house SEC-SAXS

Ovalbumin experimental SAS data
PDB model
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 24
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 55 nm3

SASDE45 – Conalbumin monomer from in-house SEC-SAXS

Ovotransferrin experimental SAS data
PDB model
Sample: Ovotransferrin monomer, 76 kDa Gallus gallus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 24
RgGuinier 3.0 nm
Dmax 10.1 nm
VolumePorod 100 nm3

SASDE55 – Apoferritin monomer from in-house SEC-SAXS

Ferritin light chain experimental SAS data
PDB model
Sample: Ferritin light chain 24-mer, 476 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 24
RgGuinier 5.1 nm
Dmax 12.3 nm
VolumePorod 685 nm3

SASDE65 – Bovine serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 92 nm3

SASDE75 – Bovine serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 104 nm3

SASDE85 – Bovine serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 85 nm3

SASDE95 – Bovine serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
RgGuinier 2.9 nm
Dmax 8.6 nm
VolumePorod 74 nm3

SASDEA5 – Bovine serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 25
RgGuinier 2.8 nm
Dmax 9.6 nm
VolumePorod 78 nm3

SASDEB5 – Bovine serum albumin from in-house SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 May 4
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 99 nm3

SASDEC5 – Bovine serum albumin dimer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L, Copenhagen University, Department of Drug Design and Pharmacology on 2018 Jan 23
RgGuinier 4.1 nm
Dmax 13.8 nm
VolumePorod 200 nm3

SASDED5 – Bovine serum albumin monomer from synchrotron SEC-SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS + 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 14
RgGuinier 2.7 nm
Dmax 8.7 nm
VolumePorod 84 nm3

SASDEE5 – Bovine serum albumin from synchrotron SAXS

Bovine serum albumin experimental SAS data
PDB model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 85 nm3