A Metastable Contact and Structural Disorder in the Estrogen Receptor Transactivation Domain

Peng Y, Cao S, Kiselar J, Xiao X, Du Z, Hsieh A, Ko S, Chen Y, Agrawal P, Zheng W, Shi W, Jiang W, Yang L, Chance M, Surewicz W, Buck M, Yang S, Structure (2018) DOI

SASDEE2 – The N-terminal domain of estrogen receptor alpha

The N-terminal domain of estrogen receptor alpha
MWexperimental 20 kDa
MWexpected 20 kDa
log I(s) 9.21×100 9.21×10-1 9.21×10-2 9.21×10-3
The N-terminal domain of estrogen receptor alpha small angle scattering data  s, nm-1
ln I(s)
The N-terminal domain of estrogen receptor alpha Guinier plot ln 9.21×100 Rg: 3 nm 0 (3 nm)-2 s2
(sRg)2I(s)/I(0)
The N-terminal domain of estrogen receptor alpha Kratky plot 1.104 0 3 sRg
p(r)
The N-terminal domain of estrogen receptor alpha pair distance distribution function Rg: 3.1 nm 0 Dmax: 10 nm

Data validation


Fits and models


log I(s)
 s, nm-1
The N-terminal domain of estrogen receptor alpha CUSTOM IN-HOUSE model

log I(s)
 s, nm-1
The N-terminal domain of estrogen receptor alpha CUSTOM IN-HOUSE model

log I(s)
 s, nm-1
The N-terminal domain of estrogen receptor alpha CUSTOM IN-HOUSE model

Synchrotron SAXS data from solutions of the N-terminal domain of estrogen receptor alpha in 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH 7.4 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source (NSLS-II; Brookhaven, NY, USA) using a Pilatus3 S 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.09 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 10°C. Three successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The N-terminal domain of estrogen receptor alpha (ER-NTD)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   20.2 kDa
 
UniProt   P03372 (1-184)
Sequence   FASTA